Teledyne 10 Manuel d'utilisateur

BioOptix™ 10Installation and Operation Guide#69-5203-150 Copyright © 2004 Teledyne Isco, Inc.All rights reserved.Revision B, September 20, 2005

BioOptix™ 10Table of Contentsviii3.8 Post-experiment Operation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3

BioOptix™ 10Table of ContentsixList of Figures1-1 BioOptix 10 front panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

BioOptix™ 10Table of ContentsxList of Tables1-1 BioOptix 10 Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

1-1BioOptix™ 10Section 1 Introduction1.1 Product Overview The BioOptix™ 10 system performs automated protein purifi-cation on as many as ten liquid sa

BioOptix™ 10Section 1 Introduction1-21.2 System Specifications Table 1-1 lists the BioOptix 10 specifications. Table 1-1 BioOptix 10 SpecificationsDi

BioOptix™ 10Section 1 Introduction1-31.3 System Controls and Connectors1.3.1 BioOptix 10 Front Panel ControlsFigure 1-1 BioOptix 10 front panel13452T

BioOptix™ 10Section 1 Introduction1-41.3.2 BioOptix 10 Left-side Controls and ConnectionsFigure 1-2 BioOptix 10 left-side connections1234576Table 1-3

BioOptix™ 10Section 1 Introduction1-51.3.3 BioOptix 10 Right-side ConnectionsFigure 1-3 BioOptix 10 right-side connections1Table 1-4 BioOptix 10 Righ

BioOptix™ 10Section 1 Introduction1-61.3.4 BioOptix 10 Rear Panel Controls and ConnectionsFigure 1-4 BioOptix 10 rear panel controls and connections1

2-1BioOptix™ 10Section 2 InstallationThis section provides instructions for installing the system andpreparing it for operation. The BioOptix 10 syste

BioOptix™ 10Section 2 Installation2-2To keep tubing length at a minimum, buffer, sample, and wastecollection containers should be placed to the left

BioOptix™ 10Section 2 Installation2-3Figure 2-2 Accessing pump and column module internal connectionsTo complete the connections:1. Release the two s

BioOptix™ 10Section 2 Installation2-43. While facing the back of the column module, locate the two liquid tubing bundles secured to the lower-left in

BioOptix™ 10Section 2 Installation2-55. Connect the orange connector labeled BD3 P110 to P110 on the Power Drive Circuit Board (Figure 2-6).Figure 2-

BioOptix™ 10Section 2 Installation2-67. Plug the black power cord from the pump module into the receptacle at the lower part of the column module (Fi

BioOptix™ 10Section 2 Installation2-710. Connect the tubing from the column module to the pump module. Refer to Figure 2-10. a. Remove the two thumbs

BioOptix™ 10Section 2 Installation2-8Figure 2-11 Aligning the fasteners Figure 2-12 Column clamps inserted

BioOptix™ 10Section 2 Installation2-912. Ensure the Power Switch is in the Off (“0”) position. Then, connect the AC power cord to the back of the Pum

BioOptix™ 10Section 2 Installation2-105. Connect the AC power cord to the back of the Foxy 200. Connect the other end of the power cord to an outlet

BioOptix™ 10Section 2 Installation2-11Figure 2-14 Buffer assembly routes a common solution to all ten channelsFigure 2-15 Buffer assemblies installed

ForewordThis instruction manual is designed to help you gain a thorough understanding of theoperation of the equipment. Teledyne Isco recommends that

BioOptix™ 10Section 2 Installation2-12Buffer B – To form gradient buffers during purification, connectBuffer B solution(s) to these ten inlet ports.

BioOptix™ 10Section 2 Installation2-13Figure 2-16 Foxy 200 tubing connections Refer to Figure 2-16 and complete the tubing connectionsbetween the Bio

BioOptix™ 10Section 2 Installation2-142.2.6 Windows PC ConnectionThe Windows® personal computer (PC) must meet the followingminimum requirements: The

BioOptix™ 10Section 2 Installation2-15❍ Rack Change Bell – When this option is enabled, the system provides an audible signal while it is waiting for

BioOptix™ 10Section 2 Installation2-166. Ensure that each channel on the BioOptix 10 has 0.125" OD bypass tubing between the Column Inlet and Ou

BioOptix™ 10Section 2 Installation2-17Figure 2-20 BioOptix 10 Manual Hardware Control window9. In the Columns section of this window, check each chan

BioOptix™ 10Section 2 Installation2-18

3-1BioOptix™ 10Section 3 OperationThis section provides an introduction to preparing the systemand creating files necessary for a purification run. Th

BioOptix™ 10Section 3 Operation3-23.3 BioOptix 10 Software NoteRefer to On-line Help for specific BioOptix 10 software operat-ing instructions.3.3.1

BioOptix™ 10Section 3 Operation3-3NoteThe percent B value is the amount of Buffer B solution mixedwith Buffer A. A value of zero percent B (0 %B) pum

BioOptix™ 10Section 3 Operation3-4The default method file contains five blocks that are appropriatefor use with affinity column purification:• Equili

BioOptix™ 10Section 3 Operation3-5Pausing at the end of a block allows for buffer changes orother mechanical adjustments before proceeding with thene

BioOptix™ 10Section 3 Operation3-63.5.3 Wash Settings This block flushes compounds that did not bind to the affinitymedia. The Wash tab is a fluid bl

BioOptix™ 10Section 3 Operation3-7• Collect Effluent – Select the desired action for effluent fluid as it comes off the column. If Ye s is selected,

BioOptix™ 10Section 3 Operation3-83.6.1 Setting up the ExperimentBefore proceeding, determine which BioOptix 10 channel posi-tions will be used durin

BioOptix™ 10Section 3 Operation3-93.6.3 Saving the Experiment FileSave the experiment by selecting the File>Save menu command.Use a descriptive fi

BioOptix™ 10Section 3 Operation3-10Figure 3-9 Pause at end of blockIf the block is set to collect effluent (Figure 3-4), the fraction col-lector will

BioOptix™ 10Section 3 Operation3-11The Fast Forward button is active after clicking the Stop button.The Fast Forward button allows you to skip to the

BioOptix™ 10Section 3 Operation3-123. Select a wash fluid. The wash fluid must be compatible with the buffer system and of sufficient strength to dis

BioOptix™ 10Section 3 Operation3-133.8.3 AutoReports After the run the BioOptix 10 can automatically print the purifi-cation run results or save the

BioOptix™ 10SafetyiiiBioOptix™ 10Safet yGeneral Warnings Before installing, operating, or maintaining this equipment, it isimperative that all hazards

BioOptix™ 10Section 3 Operation3-14

4-1BioOptix™ 10Section 4 System Maintenance4.1 Periodic Maintenance The BioOptix 10 system’s internal components have beenselected for long life and l

BioOptix™ 10Section 4 System Maintenance4-2The BioOptix 10 system pumps the wash fluid, flushing theBuffer In lines, gradient formation components, p

BioOptix™ 10Section 4 System Maintenance4-3Figure 4-2 Access the pump assemblyNoteRefer to Figure 4-3 for the following steps. 9. Remove the bottom r

BioOptix™ 10Section 4 System Maintenance4-4Figure 4-3 Pump assembly componentsFigure 4-4 Attach seal to push rod10-32 × 3/8" Pan Head Screw#10 I

BioOptix™ 10Section 4 System Maintenance4-517. Hold the push rod upside-down and insert it into the open-ing on the seal. Apply downward force until

BioOptix™ 10Section 4 System Maintenance4-6CAUTIONHot surfaces may cause burns. The UV lamp and nearby sur-faces are hot. To remove the lamp:1. Remov

BioOptix™ 10Section 4 System Maintenance4-7Replacing the lamp To install the new lamp:CAUTIONContaminants on the glass bulb will shorten the life spa

BioOptix™ 10Section 4 System Maintenance4-84.5.2 Leaks If leaks occur or if solid material is observed around a fitting,determine the source of the

BioOptix™ 10Section 4 System Maintenance4-9WARNINGRisk of damage to eyesight and skin. Wear ultraviolet (UV) light eye protection and protect skin fr

BioOptix™ 10SafetyivAVERTISSEMENTCe système peut utiliser des dissolvants organiques inflammables. Pour réduire le péril qui peut être causé par l&apo

BioOptix™ 10Section 4 System Maintenance4-104.6 Service Department If you have a problem with the instrument or need parts infor-mation, contact Isc

A-1BioOptix™ 10Appendix A Method TemplatesWhen creating a new method, the BioOptix 10 software con-figures the blocks of the method file according to

BioOptix™ 10Appendix A Method TemplatesA-22. From Notepad’s menu select File>Open. 3. Change the Files of type pull-down list to All files. 4. Use

BioOptix™ 10Appendix A Method TemplatesA-35. Check the box labeled Always use this program to open these files.6. Click OK. After doing so, double-cl

BioOptix™ 10Appendix A Method TemplatesA-4Additional rules should be observed and are described in the fol-lowing sections. A.2.2 [Blocks] The count

B-1BioOptix™ 10Appendix B Fluid Path DiagramsThis appendix contains simplified diagrams of the plumbing andvalves internal to the BioOptix 10 system.

BioOptix™ 10Appendix B Fluid Path DiagramsB-2While the system is running a fluid-type block:• The Buffer valve switches between buffers A and B to pr

BioOptix™ 10Appendix B Fluid Path DiagramsB-3Manual injections do not operate the pump. The system pausesfor the operator to turn the Mode valve to t

BioOptix™ 10Appendix B Fluid Path DiagramsB-4B.4 Pump Flush A Pump Flush should be done whenever the system will be idlefor more than a few hours. (S

BioOptix™ 10Appendix B Fluid Path DiagramsB-5Figure B-7 BioOptix 10 Plumbing Diagram

BioOptix™ 10SafetyvHazard Symbols The equipment and this manual use symbols used to warn ofhazards. The symbols are explained below.Hazard SymbolsWarn

BioOptix™ 10Appendix B Fluid Path DiagramsB-6

C-1BioOptix™ 10Appendix C Replacement Parts ListsReplacement parts are called out in illustrations in this section.Reference the call-outs in the acc

BioOptix™ 10Appendix C Replacement Parts ListsC-2

13BioOptix™ 10Replacement Parts ListsC-313BioOptix™ 10Replacemen t Parts ListsC.1 Detector Assembly

13BioOptix™ 10Replacement Parts ListsC-4

13BioOptix™ 10Replacement Parts ListsC-5

13BioOptix™ 10Replacement Parts ListsC-6

13BioOptix™ 10Replacement Parts ListsC-7C.2 Column Module

13BioOptix™ 10Replacement Parts ListsC-8

13BioOptix™ 10Replacement Parts ListsC-9

BioOptix™ 10Safetyvi

13BioOptix™ 10Replacement Parts ListsC-10

13BioOptix™ 10Replacement Parts ListsC-11C.3 Pump Module

13BioOptix™ 10Replacement Parts ListsC-12

13BioOptix™ 10Replacement Parts ListsC-13

13BioOptix™ 10Replacement Parts ListsC-14

Compliance StatementsCE Declaration of ConformityDECLARATION OF CONFORMITY Application of Council Directive: 89/336/EEC – The EMC Directive 73/23/

War ran ty In the event of instrument problems, always contact the Teledyne Isco Service Department, as problems can often be diagnosed and corrected

viiBioOptix™ 10 Table of ContentsSection 1 Introduction1.1 Product Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .